| ID |
TKG 0815 |
| Cell name |
PC12-P1F1 |
| Data accepted |
August, 2024. |
| Animal |
Rat. |
| Scientific name |
Rattus norvegicus. |
| Sex |
|
| Case history |
|
| Tissue |
Pheochromocytoma. |
| Genetics |
|
| Life span |
Infinite. |
| Morphology |
|
| Medium |
DMEM (high glucose) + 5% FBS + 5% HS (Gibco 16050-130). |
| Passage method |
0.05% trypsin / 0.02% EDTA-PBS. |
| Characteristics |
PC12-derived subclone. PC12-P1F1 is hypersensitive for neuritogenesis to
temperature-controlled repeated thermal stimulation (TRTS). BMP4- or TRTS-induced
neuritogenesis is increased in PC12-P1F1 cells compared to parental PC12
cells. |
| Temperature |
37℃ |
| CO2 concentration |
5% |
| Subculture frequency |
1:3, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Established by |
Kudo T. (Division of Oral Physiology, Tohoku University Graduate School
of Dentistry.) |
| References |
Int J Mol Sci. 2020 Nov 7;21(21):8356(PMID: 33171774). |
| References by users |
Int J Mol Sci. 2022 Dec 9;23(24):15602(PMID: 36555248). |
| Comments |
|
| Deposited by |
Kudo T. (Division of Oral Physiology, Tohoku University Graduate School
of Dentistry.) |
| Restriction |
|
| Photo |
 |
