| ID |
TKG 0791 |
| Cell name |
M14-10 |
| Data accepted |
February, 2019. |
| Animal |
Mouse x Rat
|
| Scientific name |
|
| Sex |
|
| Case history |
|
| Tissue |
B cell hybridoma |
| Genetics |
|
| Life span |
|
| Morphology |
Lymphoblast-like |
| Medium |
RPMI1640 + 10% FBS |
| Passage method |
Dilution. |
| Characteristics |
Wistar rats were immunized with mouse CD14-expressing NRK-52E transfected
cells. Mouse - Rat hybridoma producing rat monoclonal antibody, IgG2b,
kappa. HAT resistance. This cell line can be used for immunoprecipitation
and flow cytometry. No cross-reactivity with human CD14. Transplantation
into C.B-17/Icr-scid/scid mice induces ascites. |
| Temperature |
37℃ |
| CO2 concentration |
5% |
| Subculture frequency |
1:1 ~ 1:9, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Established by |
Tsukamoto, H. |
| References |
Int Immunol. 2010 22(4):271-80(PMID: 20133493). |
| References by users |
|
| Comments |
|
| Deposited by |
Tsukamoto, H. |
| Restriction |
C: A prior written permission of the originator is necessary. |
| Photo |
 |
