ID	TKG 0789
Cell name	hLp4
Data accepted	February, 2019.
Animal	Mouse x Rat
Scientific name
Sex
Case history
Tissue	B cell hybridoma
Genetics
Life span
Morphology	Lymphoblast-like
Medium	RPMI1640 +10% FBS or Hybridoma SFM.
Passage method	Dilution.
Characteristics	Wistar rats were immunized with human LBP N-terminal domain (aa1-212) recombinant protein named human lipopolysaccharide (LPS) - binding protein (LBP). Mouse - Rat hybridoma producing rat monoclonal antibody, IgM, kappa. HAT resistance. This cell line can be used for ELISA and western blotting. This inhibits LBP-LPS binding.
Temperature	37℃
CO2 concentration	5%
Subculture frequency	1:1 ~ 1:4, twice a week.
Classification
Mycoplasma	PCR: -, Hoechst DNA staining: -
Established by	Tsukamoto, H.
References
References by users
Comments
Deposited by	Tsukamoto, H.
Restriction	C: A prior written permission of the originator is necessary.
Photo