| ID |
TKG 0788 |
| Cell name |
hLp1 |
| Data accepted |
February, 2019. |
| Animal |
Mouse x Rat
|
| Scientific name |
|
| Sex |
|
| Case history |
|
| Tissue |
B cell hybridoma. |
| Genetics |
|
| Life span |
|
| Morphology |
Lymphoblast-like. |
| Medium |
RPMI1640 +10% FBS or Hybridoma SFM. |
| Passage method |
Dilution. |
| Characteristics |
Wistar rats were immunized with human LBP N-terminal domain (aa1-212) recombinant
protein named human lipopolysaccharide (LPS) - binding protein (LBP). Mouse
- Rat hybridoma producing rat monoclonal antibody, IgM, kappa. HAT resistance.This
cell line can be used for ELISA and western blotting. This inhibits LBP-LPS
binding. |
| Temperature |
37℃ |
| CO2 concentration |
5% |
| Subculture frequency |
1:4 ~ 1:9, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Established by |
Tsukamoto, H. |
| References |
|
| References by users |
|
| Comments |
|
| Deposited by |
Tsukamoto, H. |
| Restriction |
C: A prior written permission of the originator is necessary. |
| Photo |
 |
