細胞株の詳細データ
TKG 0766：： GFP2
| ID | TKG 0766 |
| Cell name | GFP2 |
| Data accepted | January, 2019. |
| Animal | Mouse x Rat |
| Scientific name | |
| Sex | |
| Case history | |
| Tissue | B cell hybridoma. |
| Genetics | |
| Life span | |
| Morphology | Lymphoblast-like. |
| Medium | RPMI1640 + 10% FBS |
| Passage method | Dilution. |
| Characteristics | Wistar rats were immunized in the foot pads with EGFP. Mouse-Rat hybridoma
producing rat monoclonal antibody, IgG2a, kappa. HAT resistance. This cell line can be used for immunoprecipitation for antigen with EGFP in C-terminal and FACS. Transplantation into C.B-17/Icr-scid/scid mice induces ascites. |
| Temperature | 37℃ |
| CO2 concentration | 5% |
| Subculture frequency | 1:4 ~ 1:10, twice a week. |
| Classification | |
| Mycoplasma | PCR: -, Hoechst DNA staining: - |
| Established by | Tsukamoto, H. |
| References | J Biol Chem.2018 293(26):10186-10201.(PMID: 29760187) |
| References by users | |
| Comments | |
| Deposited by | Tsukamoto, H. |
| Restriction | C: A prior written permission of the originator is necessary. |
| Photo |   (x100) (x200) |