| ID |
TKG 0759 |
| Cell name |
mTX12 |
| Data accepted |
January, 2019. |
| Animal |
Mouse x Rat
|
| Scientific name |
|
| Sex |
|
| Case history |
|
| Tissue |
B cell hybridoma. |
| Genetics |
|
| Life span |
|
| Morphology |
Lymphoblast-like. |
| Medium |
RPMI1640 + 10% FBS |
| Passage method |
Dilution. |
| Characteristics |
Immune animal is Wistar rat. Antigen is mouse testicular homogenate named
TEX101. Mouse-Rat hybridoma producing rat monoclonal antibody, IgG2a. HAT
resistance. This cell line can be used for immunoprecipitation, flow cytometry
and non-reducing western blotting. It may also be used for immunohistochemical
staining. Transplantation into C.B-17/Icr-scid/scid mice induces ascites.
|
| Temperature |
37℃ |
| CO2 concentration |
5% |
| Subculture frequency |
1:4, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Established by |
Tsukamoto, H. |
| References |
Sci Rep.2016 6:23616 (PMID: 270005865) |
| References by users |
|
| Comments |
|
| Deposited by |
Tsukamoto, H. |
| Restriction |
C: A prior written permission of the originator is necessary. |
| Photo |
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