細胞株の詳細データ
TKG 0750：： Replic
| ID | TKG 0750 |
| Cell name | Replic |
| Data accepted | August, 2019. |
| Animal | Mouse, C57BL/6 |
| Scientific name | Mus musculus. |
| Sex | Male. |
| Case history | |
| Tissue | Kidney, REP (renal erythropoietin-producing) cells. |
| Genetics | |
| Life span | Infinite. |
| Morphology | Fibroblast-like |
| Medium | DMEM high glucose+ 10% FBS |
| Passage method | Accutase (Nakarai 12679-54) or 0.05% trypsin. |
| Characteristics | The immortalized cell line prepared by the exogenous expression of oncogenic
H-RAS (G12V). tdTomato-positive. High-level TGFβ expression. This cell line was isolated from Epo(GFP/wt)Tg(3.3kEpo)Tg(EpoCre)Rosa26(LSLtdTomato) genotype mice. A culture submitted to Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by treatments with BM Cyclin and MC210. |
| Temperature | 37℃ |
| CO2 concentration | 5% |
| Subculture frequency | 1:5 ~ 1:10, twicw a week |
| Classification | |
| Mycoplasma | PCR: -, Hoechst DNA staining: - |
| Established by | Suzuki, N. |
| References | Sci Rep 9(1):11254(2019). PMID: 31375751. |
| References by users | |
| Comments | |
| Deposited by | Suzuki,N. |
| Restriction | C: A prior written permission of the originator is necessary. |
| Photo |  |