細胞株の詳細データ
TKG 0731 ：： R-3
| ID | TKG 0731 |
| Cell name | R-3 |
| Data accepted | Sept, 2017. |
| Animal | Rat (Donryu). |
| Scientific name | Rattus norvegicus. |
| Sex | M |
| Case history | |
| Tissue | Lymph node with metastasis from esophageal tumor. |
| Genetics | |
| Life span | Infinite. |
| Morphology | Squamous cell carcinoma. |
| Medium | Eagle MEM + 10% FBS. |
| Passage method | 0.25% trypsin. |
| Characteristics | This cell line was established from squamous cell carcinoma of the esophagus
induced in Donryu rats by N-amyl-N-Methyl-nitrosamine. Transplantable tumor
cell line (nude mouse, sc). A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by treatments with BM cyclin and MC210. |
| Temperature | 37℃ |
| CO2 concentration | 5% |
| Subculture frequency | 1:10, twice/week. |
| Classification | |
| Mycoplasma | PCR: -, Hoechst DNA staining: - |
| Established by | Iizuka, T and Ichimura, S. |
| References | Jpn. J. Clin. Oncol. 1981, 11 (3), 487-494. |
| References by users | |
| Comments | |
| Deposited by | Nishida, C. (Genome Dynamics Research Center, Faculty of Science, Hokkaido University.) |
| Restriction | |
| Photo |  (x100) |