細胞株の詳細データ
TKG 0727：： NCB20
| ID | TKG 0727 |
| Cell name | NCB20 |
| Data accepted | July, 2015 |
| Animal | Mouse x Chinese hamster |
| Scientific name | Mus musculus; Cricetulus griseus. |
| Sex | |
| Case history | |
| Tissue | N18TG-2 mouse neuroblastoma x Chinese hamster embryonic brain cells |
| Genetics | |
| Life span | Infinite |
| Morphology | |
| Medium | DMEM + HAT + 5% FBS |
| Passage method | 1:5-1:20 with PBS (-) or 0.05% trypsin. |
| Characteristics | Transplantable tumor cell line. Cholinergic. |
| Temperature | 37℃ |
| CO2 concentration | 10% |
| Subculture frequency | Twice a week. |
| Classification | |
| Mycoplasma | PCR: -, Hoechst DNA staining: - |
| Species identification by nested PCR | Only the mouse-specific band was observed. (PMID: 17516125) |
| Established by | Marshall Nirenberg. |
| References | Neurochemistry International 61 (2012) 821-827 (PMID: 22414530), Cancer Genetics and Cytogenetics 16, 219-227(1985) (PMID:397859), Folia phermacol. japon. 77, 99-113 (1981) |
| References by users | |
| Comments | |
| Deposited by | Higashida, H. (Research Center for Child Mental Development, Kanazawa University) |
| Restriction | |
| Photo |  |