| ID |
TKG 0725 |
| Cell name |
Seki |
| Data accepted |
December, 2014 |
| Animal |
Human. |
| Scientific name |
Homo sapiens. |
| Sex |
M |
| Case history |
|
| Tissue |
Ewing's sarcoma. |
| Genetics |
Translocation of t(11;22)(q24;q12), EWSR1-Fli1. |
| Life span |
Infinite. |
| Morphology |
|
| Medium |
RPMI1640 + 10% FBS. |
| Passage method |
0.05% trypsin/EDTA, collagen coated dish. |
| Characteristics |
Transplantable tumor cell line (subcutaneously, Nude mouse, SCID). |
| Temperature |
37℃ |
| CO2 concentration |
5% |
| Subculture frequency |
1:5-1:10, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: ± |
| Established by |
Nojima, T. |
| References |
Igaku no ayumi. 145(7):511-512. Human Cell. 2:192-193(PMID: 2486607). Nagoya Med J. 35:169-180. Genes Chromosome Cancer. 13:126-132(PMID: 7542907). |
| References by users |
|
| Comments |
|
| Deposited by |
Nojima, T. |
| Restriction |
|
DNA Profile
(STR-PCR) |
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
| 10, 12 |
8, 12 |
10, 13 |
12, 13 |
14, 18 |
6, 9 |
XY |
8 |
10, 12 |
|
| Photo |
 |
