細胞株の詳細データ
TKG 0721 ：： Hepa1-3xFlag-AhR-HA-pCEP4(CAG)H12C1
| ID | TKG 0721 |
| Cell name | Hepa1-3xFlag-AhR-HA-pCEP4(CAG)H12C1 |
| Data accepted | March, 2014 |
| Animal | Mouse C56BL |
| Scientific name | Mus musculus. |
| Sex | |
| Case history | |
| Tissue | Liver |
| Genetics | Stable Hepa1c1c7 cl19E4 mutant transfected with 3xFlag-HA-tagged aryl hydrocarbon receptor (AhR) gene. |
| Life span | Infinite |
| Morphology | Fibroblast-like. |
| Medium | DMEM + 5% FBS |
| Passage method | 0.25% trypsin/EDTA |
| Characteristics | Resistant to 150㎍/ml hygromycin B. Useful for analysis of AhR transcription
factor complex. A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210. |
| Temperature | 37℃ |
| CO2 concentration | 5% |
| Subculture frequency | 1:10, twice a week. |
| Classification | |
| Mycoplasma | PCR: -, Hoechst DNA staining: - |
| Established by | Ebina, M. |
| References | Ebina M, et al. Biochim. Biophys.Acta,1809,176 (2011) |
| References by users | |
| Comments | |
| Deposited by | Kikuchi, H. |
| Restriction | |
| Photo |  |