細胞株の詳細データ

TKG 0715 ：： ISO-HAS-B

ID	TKG 0715
Cell name	ISO-HAS-B
Data accepted
Animal	Human.
Scientific name	Homo sapiens.
Sex	M
Case history
Tissue	Hemangiosarcoma arising on scalp
Genetics
Life span	Infinite
Morphology	Polygonal to cobblestone appearance at confluence.
Medium	DMEM (high glucose) + 10% FBS.
Passage method	0.25% Trypsin-EDTA
Characteristics	Angiosarcoma. Endothelial cell like. Poorly differentiated. This cell line displays endothelial cell-like phenotype such as Dil-Ac-LDL uptake and UEA-1lectin-binding. Weakly positive for von Willebrand factor. CD31 positive. HLA-ABC positive. HLA-DR negative. A point mutation in p 53 is reported. This cell line is ISO-HAS naturalized to DMEM. Slow-growing.
CO2 concentration	5%
Subculture frequency	1:1, twice a week.
Classification
Mycoplasma	PCR: -, Hoechst DNA staining: -
Established by	Masuzawa M. (Department of Regulation Biochemistry, Kitasato University School of Allied Sciences)
References	Int. J. Cancer 81: 305-308, 1999. PMID: 10188735, J. Dermatol. Sci. 16: 91-98, 1998. PMID: 9459120
References by users	Anticancer Res. 2019 Mar;39(3):1143-1150(PMID: 30842143), Exp Dermatol. 2023 Jan;32(1):50-59(PMID: 36168721).
Comments	When you begin to culture this cell line, please be careful not to lower cell density. 1tube --> 60mm cell culture dish x 2 or smaller scale
Deposited by	Masuzawa M. (Department of Regulation Biochemistry, Kitasato University School of Allied Sciences)
Restriction
DNA Profile (STR-PCR)	D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO 10 8 10,11 11 14 7,9 XY 8, 11 11
Photo