細胞株の詳細データ
TKG 0666 ：： B16 C2W
| ID | TKG 0666 |
| Cell name | B16 C2W |
| Data accepted | |
| Animal | Mouse (C57BL/6J). |
| Scientific name | Mus musculus. |
| Sex | |
| Case history | |
| Tissue | Melanoma. |
| Genetics | |
| Life span | Infinite |
| Morphology | Epithelial-like. |
| Medium | RPMI-1640 + 10% FBS. |
| Passage method | 0.05% Trypsin. |
| Characteristics | Mouse melanoma, melanin production (-). This tissue culture cell line was established from a cell line maintained by in vivo transplantation. Conditions for transplantation, sc:80-100:20-30 (transplantation site : transplantation rate : survival days). The cell line was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210. |
| CO2 concentration | 5% |
| Subculture frequency | 1:4, 2 ~ 3 times / week. |
| Classification | |
| Mycoplasma | PCR: -, Hoechst DNA staining: - |
| Isozyme analysis | |
| Established by | Oikawa, A |
| References | Cell differentiation, 1, 149-155, 1972.PMID: 4209534. |
| References by users | Mol Cell Biol. 2019 Aug 12;39(17):e00154-19(PMID: 31208979). |
| Comments | |
| Deposited by | Oikawa, A. (RITC). |
| Restriction | |
| Photo |  Photo(x100) |