細胞株の詳細データ
TKG 0661 ：： SMAP1dm
| ID | TKG 0661 |
| Cell name | SMAP1dm (SMAP1-deficient macrophage) |
| Data accepted | |
| Animal | SMAP1(-/-) mouse(C57/BL6). |
| Scientific name | Mus musculus. |
| Sex | F. |
| Case history | |
| Tissue | Bone marrow. |
| Genetics | |
| Life span | Infinite. |
| Morphology | |
| Medium | complete RPMI-1640 + 10% FBS+PC/SM |
| Passage method | 0.05% Trypsin + 1mM EDTA |
| Characteristics | Immunophenotype are Mac1+, c-kitlow, CD34low, CD71low, Gr1-, Ter119-, CD3- and B220-. Expresses soluble form of SCF. Differentiated cell line derived from leukemia cell of monocytic leukemia. c-Kit inhibitors (imatinib, ISCK03) decrease cell viability. This cell line is killed by 4μg/ml of puromycin. |
| CO2 concentration | 5% |
| Subculture frequency | 1:5, twice a week. |
| Classification | |
| Mycoplasma | PCR: -, Hoechst DNA staining: - |
| Isozyme analysis | LD |
| Established by | Kon S. (IDAC, Tohoku Univ.) |
| References | Kon S, Tanabe K, Watanabe T, Sabe H, Satake M. Exp Cell Res., 314(7):1415-1428 (2008). |
| References by users | |
| Comments | |
| Deposited by | Satake M. (IDAC, Tohoku Univ.) |
| Restriction | |
| Photo |  Photo(x100) |