細胞株の詳細データ
TKG 0622 ：： TBR10-1
| ID | TKG 0622 |
| Cell name | TBR10-1 |
| Data accepted | March 2000. |
| Animal | C57BL6 SV40(tsA58) transgenic mouse. |
| Scientific name | |
| Sex | |
| Case history | |
| Tissue | Bone marrow. |
| Genetics | |
| Life span | Infinite |
| Morphology | Fibroblast-like |
| Medium | RITC80-7, 2% FBS, 10ng/ml EGF, 10ug/ml Tf, & 1ug/ml Insulin. |
| Passage method | 0.25% trypsin-EDTA-PBS, 33oC culture. |
| Characteristics | Bone marrow fibroblast. Optimal culture temperature 33oC, but culturable at 37oC, using type I collagen 6 well plate (Sumitomo bakelite MS-0006K plate). |
| CO2 concentration | 5% |
| Subculture frequency | 1:5, twice a week. |
| Classification | |
| Mycoplasma | PCR: -, Hoechst DNA staining: ± |
| Isozyme analysis | LD, NP |
| Established by | Dr. Nobuaki Yanai (Institute of DEvel. Aging and Cancer). Anyone wants to use, contact Prof. Obinata. |
| References | J Cell Physiol. 1995 Jul;164(1):55-64 PMID: 7790397 |
| References by users | |
| Comments | |
| Deposited by | Dr. Yanai |
| Restriction | In publishing the research results obtained by use this cell line, a citation of the following literature is necessary. J Cell Physiol. 1995 Jul;164(1):55-64 PMID: 7790397 |
| Photo |  Photo(x100) |