| ID |
TKG 0593 |
| Cell name |
ISO-HAS |
| Data accepted |
|
| Animal |
Human |
| Scientific name |
Homo sapiens |
| Sex |
|
| Case history |
|
| Tissue |
Hemangiosarcoma arising on scalp |
| Genetics |
|
| Life span |
Infinite |
| Morphology |
Polygonal to cobblestone appearance at confluence. |
| Medium |
DMEM (high glucose) + 15% FBS + ISOS-1 conditioned medium 50%.*The ISOS-1
conditioned medium was harvested after confluent ISOS-1 cells were cultured
with medium for 3 to 4 days and filtered by a 0.22-μm Bottle Top Filter. |
| Passage method |
0.25% Trypsin-EDTA |
| Characteristics |
Angiosarcoma. Endothelial cell like. Poorly differentiated. This cell line
displays endothelial cell-like phenotype such as Dil-Ac-LDL uptake and
UEA-1lectin-binding. Weakly positive for von Willebrand factor. CD31 positive.HLA-ABC
positive. HLA-DR negative. A point mutation in p 53 is reported.This cell
line was established from 84-year old male. Transplantable to SCIDmice.
Restriction: Agreement from Dr. Masuzawa required. |
| CO2 concentration |
5% |
| Subculture frequency |
1:1 ~ 1:2, once or twice / week |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Isozyme analysis |
LD |
| Established by |
Masuzawa, M. (Kitasato Univ. Dermatology) |
| References |
Int. J. Cancer, 81, 305-308, 1999.PMID: 10188735 |
| References by users |
Microvasc Res. 2013 Jul:88:25-31(PMID: 23567873), Cancer Chemother Pharmacol. 2014 Feb;73(2):259-70(PMID: 24253175), J Dermatol Sci. 2015 Apr;78(1):44-50(PMID: 25726712). |
| Comments |
|
| Deposited by |
Masuzawa, M. (Kitasato Univ. Dermatology) |
| Restriction |
|
DNA Profile
(STR-PCR) |
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
| 10 |
8 |
10,11 |
11 |
14 |
7,9 |
XY |
8,11 |
11 |
|
| Photo |
 |
