| ID |
TKG 0571 |
| Cell name |
A20 (mouse B lymphoma) |
| Data accepted |
|
| Animal |
BALB/c mouse |
| Scientific name |
Mus musculus |
| Sex |
|
| Case history |
|
| Tissue |
|
| Genetics |
|
| Life span |
Infinite. |
| Morphology |
Lymphoblast-like |
| Medium |
RPMI-1640 +10% FBS |
| Passage method |
Dilution |
| Characteristics |
BALB/c lymphoma. Established from spontaneous reticulum cell lymphoma found
in an old BALB/c AnN. These cells are IgG(+), Ia(+), Fc-R(+), IgM(-), IgA(-)
and complement receptor (-). Diploid and tumorigenic surfaci Ig, however,
cells were grown in RPMI-1640 they express a high level of surface Ig.
T cell factors and mitogens can induce these cells tosecrete IgG extracellularly.
This cell line can present both alloantigens and protein antigens. A culture
submitted to the Cell Resource Center for Biomedical Research was found
to be contaminated with mycoplasma. Progeny were cured by Treatments with
BM Cyclin and MC210. |
| CO2 concentration |
5% |
| Subculture frequency |
1:10~1:20, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Isozyme analysis |
LD |
| Established by |
|
| References |
J. Immunol., 122, 549-554, 1979(PMID: 310843)., J. Exp. Med., 155, 445-459, 1982(PMID: 6460073). |
| References by users |
Immunol. Lett., 98, 232-244, 2005(PMID: 15860223), J Immunol. 2011 Jun 15;186(12):7050-9 (PMID: 21555536). |
| Comments |
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| Deposited by |
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| Restriction |
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| Photo |
 (x100) |
