| ID |
TKG 0567 |
| Cell name |
BeWo |
| Data accepted |
|
| Animal |
Human. |
| Scientific name |
|
| Sex |
|
| Case history |
|
| Tissue |
Fetal placenta |
| Genetics |
|
| Life span |
Infinite. |
| Morphology |
Epithelial-like. |
| Medium |
F12 85% and 15% FBS or RPMI 1640 45% +5% FBS, and 50% GIT medium(Wako) |
| Passage method |
0.02% EDTA-PBS. |
| Characteristics |
Human choriocarcinoma (trophoblastic cell line) The tumor was originally
transplanted to the hamster cheek pouch by R. Hertz where it was maintained for
304 serial transfers over a period of 8 years before establishment in vitro. The
BeWo cell line has been shown to secrete hCG, polypeptide hormone, estrogen and
estradiol. |
| CO2 concentration |
5% |
| Subculture frequency |
1:4, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Isozyme analysis |
|
| Established by |
Pattillo, R.A. and Gey, G.O. |
| References |
Proc. Soc. Exp. Biol. Med., 102, 77, 1959(PMID: 14401422)., Cancer Res., 28, 1231-1236, 1968(PMID: 4299001)., Science, 159, 1467-1469, 1968(PMID: 5753554)., Ann. N. Y. Acad. Sci., 172, 288-298, 1971(PMID: 5289994).,. J. Clin. Endocrinol. Metab., 34, 59-61, 1972(PMID: 4332667). |
| References by users |
Cancer Res., 67, 3945-3954, 2007(PMID: 17440110). |
| Comments |
|
| Deposited by |
|
| Restriction |
|
DNA Profile
(STR-PCR) |
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
| 10, 11 |
9, 11 |
10, 12 |
13, 14 |
16 |
9, 9.3 |
XY |
8 |
11, 12 |
|
| Photo |
  |
