| ID |
TKG 0514 |
| Cell name |
Cos-7 |
| Data accepted |
|
| Animal |
African green monkey |
| Scientific name |
|
| Sex |
|
| Case history |
|
| Tissue |
Kidney |
| Genetics |
|
| Life span |
Infinite |
| Morphology |
Fibroblast-like |
| Medium |
D-MEM + 10% FBS |
| Passage method |
0.25% trypsin |
| Characteristics |
This cell line is a fibroblast-like cell line established from CV-1 simian
cells which were transformed by an origin-defective mutant of SV40 which codes
for wild-type T-antigen. This contains T-antigen. This cell line is suitable for
transfection. Handle as potentially biohazardous material under at least
Biosafety Level 2 containment. |
| CO2 concentration |
5% |
| Subculture frequency |
1:10～1:20, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Isozyme analysis |
LD |
| Established by |
|
| References |
Cell,
23, 175-182, 1981.PMID: 6260373 |
| References by users |
|
| Comments |
|
| Deposited by |
|
| Restriction |
|
| Photo |
 |
