| ID |
TKG 0473 |
| Cell name |
KLN205-MUC1 |
| Data accepted |
|
| Animal |
DBA/2 mouse. |
| Scientific name |
Mus musculus. |
| Sex |
|
| Case history |
|
| Tissue |
Squamous cell carcinoma. |
| Genetics |
|
| Life span |
Infinite. |
| Morphology |
Epithelial-like. |
| Medium |
E-MEM + 10% FBS (or RPMI-1640 + FBS). |
| Passage method |
0.25% trypsin. |
| Characteristics |
This cell line was established by transfecting APR-MUC1 plasmid to KLN205
(TKG 214). Remarkable expression of MUC1 antigen can be detected. |
| Temperature |
37℃ |
| CO2 concentration |
5% |
| Subculture frequency |
1:10 ~ 1:20, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Isozyme analysis |
LD |
| Established by |
T. Kaneko (Nagano Susaka Hospital). |
| References |
Cancer
Res., 38, 2084-2090, 1978.PMID: 418873 J.
Cell SCience, 100, 841-849, 1991PMID: 1814933 Proc.
Natl. Acad. Sci. USA, 84, 4831-4835, 1987.PMID: 2440031 |
| References by users |
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| Comments |
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| Deposited by |
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| Restriction |
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| Photo |
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