| ID |
TKG 0463 |
| Cell name |
P30/OHK |
| Data accepted |
June 1995. |
| Animal |
Human. |
| Scientific name |
Homo sapiens. |
| Sex |
|
| Case history |
|
| Tissue |
Peripheral blood. |
| Genetics |
|
| Life span |
Infinite. |
| Morphology |
Lymphoblast-like. |
| Medium |
RPMI-1640 + 10% FBS. |
| Passage method |
Dilute every 4 days. |
| Characteristics |
Established feom 11 y-o female with non T non B acute lymphoblastic leukemia
(ALL). This cell line shows CD5 (-), CD2 (-), Ia (+), CD20 (+), CD19 (+), J5
(+), TdT (+), EBNA (-), CD8 (+/-), PAS (+), AcPase (+). Pre B cell line. (CD8
antigen is induced by addition of TPA). |
| CO2 concentration |
5% |
| Subculture frequency |
|
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: ± |
| Isozyme analysis |
LD, NP |
| Established by |
Hirose M. |
| References |
Gann,
73, 600-605, 1982.PMID: 6983990 |
| References by users |
|
| Comments |
|
| Deposited by |
|
| Restriction |
|
DNA Profile
(STR-PCR) |
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
| 11,12 |
10 |
10, 11 |
9 |
14, 21 |
9 |
X |
10, 11 |
9, 11 |
|
| Photo |
 |
