| ID |
TKG 0457 |
| Cell name |
CoLo 205 |
| Data accepted |
April 1996. |
| Animal |
Human. |
| Scientific name |
Homo sapiens. |
| Sex |
|
| Case history |
|
| Tissue |
Colon, adenocarcinoma.
|
| Genetics |
|
| Life span |
Infinite. |
| Morphology |
Epithelial-like with suspension cells. |
| Medium |
RPMI-1640 + 10% FBS. |
| Passage method |
0.02% EDTA-PBS. |
| Characteristics |
Established from ascitic fluid of a 70-y-o male with carcinoma of colon.
CEA-producing. transplantable to SCID mouse, subcutanousl and intraperitoneally.
HLA-A 0101/0201
|
| Temperature |
37℃ |
| CO2 concentration |
5% |
| Subculture frequency |
1:4～1:10, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Established by |
T.U. Semple. |
| References |
Cancer Res., 38, 1345-1355, 1978(PMID: 565251). |
| References by users |
Proc. Natl. Acad. Sci., 96, 4874-4973, 1999(PMID: 10220386)., Proc. Natl. Acad. Sci., 99, 10718-10723, 2002(PMID: 12149448)., Biochem. J., 394, 647-656, 2006(PMID: 16241905)., Cancer Sci, 101, 1822-1827, 2010(PMID: 20500518), Cancer Med. 2013 Jun;2(3):305-15(PMID: 23930207), Curr Issues Mol Biol. 2023 Apr 20;45(4):3658-3673.(PMID: 37185762), Int J Mol Med. 2023 Feb;51(2):18.(PMID: 36660940), Int J Mol Sci. 2023 May 7;24(9):8411(PMID: 37176118). |
| Comments |
|
| Deposited by |
|
| Restriction |
|
DNA Profile
(STR-PCR) |
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
| 10, 13 |
10, 12 |
9, 10 |
12, 13 |
15 |
8, 9 |
X |
11 |
11, 12 |
|
| Photo |
 (x100) |
