| ID |
TKG 0444 |
| Cell name |
HeLa.S3 |
| Data accepted |
April, 1995 |
| Animal |
Human. |
| Scientific name |
Homo sapiens.
|
| Sex |
|
| Case history |
|
| Tissue |
Uterine. |
| Genetics |
|
| Life span |
Infinite. |
| Morphology |
Epithelial-like, mostly floating.
|
| Medium |
RPMI 1640 + 10% FBS. |
| Passage method |
0.02% EDTA-PBS.
|
| Characteristics |
A clonal derivative of the parent HeLa cell line.
|
| Temperature |
37℃ |
| CO2 concentration |
5% |
| Subculture frequency |
1:4～1:10, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Established by |
T.T. Puck. |
| References |
J.
Exp. Med., 110, 445-450, 1959.PMID: 13814142 J.
Exp. Med., 113, 467-474, 1961PMID: 13694368 . J. Exp. Med., 103, 273-284,
1956 |
| References by users |
J Nat Prod. 2017 Feb 24;80(2):499-502(PMID: 28181805). |
| Comments |
|
| Deposited by |
|
| Restriction |
|
DNA Profile
(STR-PCR) |
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
| 11, 12 |
13.3 |
8, 12 |
9, 10 |
16, 18 |
7 |
X |
8, 12 |
9, 10 |
|
| Photo |
 (x100) |
