| ID |
TKG 0438 |
| Cell name |
HuH-28 |
| Data accepted |
June, 1995.
|
| Animal |
Human.
|
| Scientific name |
Homo sapiens. |
| Sex |
|
| Case history |
|
| Tissue |
Cholangiocellular carcinoma (intrahepatic).
|
| Genetics |
|
| Life span |
Infinite.
|
| Morphology |
Spindle-shaped with small percentage of poly-gonal-shaped cells. |
| Medium |
MEM + 10% FBS. |
| Passage method |
0.25% Trypsin. |
| Characteristics |
Slow growing, doubling time is about 80 h. Not transplantable to nude mouse.
Alkaline phosphatase (+), Gamma GTP (+), beta microglubulin (+), ferritin
(+), Elastase-1 (+), tissue peptide antigen (+). A culture submitted to
the Cell Resource Center for Biomedical Research was found to be contaminated
with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
|
| Temperature |
37℃ |
| CO2 concentration |
5% |
| Subculture frequency |
1:1～1:2, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Established by |
Y. Kusaka. |
| References |
Human cell (Japanese), 1, 92-94, 1988(PMID: 2856443)., Res. Exp. Med. Berl., 188, 367-375, 1988(PMID: 2852388). |
| References by users |
Int. J. Oncol., 36, 245-254, 2010(PMID: 19956853). |
| Comments |
|
| Deposited by |
|
| Restriction |
|
DNA Profile
(STR-PCR) |
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
| 9, 12 |
9, 12 |
10, 11 |
9 |
17 |
9 |
X |
8 |
9, 12 |
|
| Photo |
 (x100) |
