| ID |
TKG 0403 |
| Cell name |
HEp-2 |
| Data accepted |
July, 1994. |
| Animal |
Human. |
| Scientific name |
Homo sapiens.
|
| Sex |
|
| Case history |
|
| Tissue |
Larynx, Epidermoid carcinoma |
| Genetics |
|
| Life span |
Infinite.
|
| Morphology |
Epithelial-like |
| Medium |
MEM + 10% FBS. |
| Passage method |
0.05% trypsin. |
| Characteristics |
This cell line was established from tumors that had been produced in
irradiated-cortisinized weanling rats after injection with epidermoid carcinoma
tissue from the larynx of a 56-y-o Caucasian male. The in vitro isolation was
accomplished in each of several mixtures of bovine amniotic fluid, embryo
extracts, human and horse sera, and balanced salt solution, and the
epithelial-like cells subsequently grew well in several types of culture media.
It is a hardy cell line which resists temperature, nutritional, and
environmental changes without loss of viability. This line supports growth of
arvoviruses, and measles virus. This line has HeLa markers. |
| Temperature |
37℃ |
| Survival |
|
| CO2 concentration |
5% |
| Subculture frequency |
1:4 ~ 1:9, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Established by |
|
| References |
Cancer Res., 14, 660, 1954(PMID: 13209540)., Cancer Res., 15, 598, 1955(PMID: 13261081)., Proc. Soc. Exp. Biol. Med., 93, 107, 1956(PMID: 13370591)., Texas Rep. Biol. Med., 15, 588, 1957(PMID: 13495958)., Ann. N.Y. Acad. Sci., 76, 497, 1958(PMID: 13627875). |
| References by users |
Gene Therapy, 10, 51-58, 2003(PMID: 12525837). |
| Comments |
|
| Deposited by |
Suzuki, M. (Otolaryngol. Tohoku Univ.).
|
| Restriction |
|
DNA Profile
(STR-PCR) |
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
| 11, 12 |
12, 13.3 |
8, 12 |
9, 10 |
16, 18 |
7 |
X |
8, 12 |
9, 10 |
|
| Photo |
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