| ID |
TKG 0347 |
| Cell name |
B16F1 |
| Data accepted |
June, 1988. |
| Animal |
Mouse (C57BL/6J). |
| Scientific name |
Mus musculus. |
| Sex |
|
| Case history |
|
| Tissue |
Melanoma. |
| Genetics |
|
| Life span |
Infinite. |
| Morphology |
Epithelial-like.
|
| Medium |
RPMI-1640 + 10% FBS. |
| Passage method |
0.25% trypsin. |
| Characteristics |
Useful for metastasis study. A culture submitted to the Cell Resource
Center for Biomedical Research was found to be contaminated with mycoplasma.
Progeny were cured by Treatments with BM Cyclin and MC210. |
| Temperature |
37℃ |
| CO2 concentration |
5% |
| Subculture frequency |
1:20, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Isozyme analysis |
LD |
| Established by |
Fidler, I. J.
|
| References |
Nature
New Biology, 242, 148-149, 1973.PMID: 4512654
|
| References by users |
|
| Comments |
|
| Deposited by |
Dep. Radiology, RITC. |
| Restriction |
|
| Photo |
 |
