| ID |
TKG 0346 |
| Cell name |
B16C2M |
| Data accepted |
|
| Animal |
Mouse (C57BL/6J).
|
| Scientific name |
Mus musculus. |
| Sex |
|
| Case history |
|
| Tissue |
Melanoma. |
| Genetics |
|
| Life span |
Infinite. |
| Morphology |
Epithelial-like.
|
| Medium |
RPMI-1640 + 10% FBS. |
| Passage method |
0.25% trypsin.
|
| Characteristics |
Melanotic, cloned twice. A culture submitted to the Cell Resource Center
for Biomedical Research was found to be contaminated with mycoplasma. Progeny
were cured by Treatments with BM Cyclin and MC210. |
| Temperature |
37℃ |
| CO2 concentration |
5% |
| Subculture frequency |
1:20, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Isozyme analysis |
LD |
| Established by |
Oikawa, A. |
| References |
Cell
differentiation, 1, 149-155, 1972.PMID: 4209534
|
| References by users |
|
| Comments |
|
| Deposited by |
Oikawa, A. (RITC).
|
| Restriction |
|
| Photo |
 |
