細胞株の詳細データ
TKG 0328 ：： CHO-K1
| ID | TKG 0328 |
| Cell name | CHO-K1 |
| Data accepted | Nov., 1987. |
| Animal | Chinese hamster. |
| Scientific name | Cricetulus griseus. |
| Sex | |
| Case history | |
| Tissue | Ovary. |
| Genetics | |
| Life span | Infinite. |
| Morphology | Epithelial-like. |
| Medium | F12 medium + 10% FBS. |
| Passage method | 0.25% trypsin. |
| Characteristics | This cell line was derived as subclone from parental CHO cell line initiated from a biopsy of an ovary of an adult hamster. This cell line has a requirement for proline. Useful for induction and isolation of nutritionally deficient mutants and for regulation of gene expression through hybridization. A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210. |
| Temperature | 37℃ |
| CO2 concentration | 5% |
| Subculture frequency | 1:10～1:20, twice a week. |
| Classification | |
| Mycoplasma | PCR: -, Hoechst DNA staining: - |
| Isozyme analysis | LD |
| Established by | Puck, T. T. |
| References | J. Exp. Med., 108, 945, 1958(PMID: 13598821)., Proc. NAS., 52, 288, 1965., Genetics, 55, 513, 1967(PMID: 6068403)., Proc. NAS., 60, 1275, 1968. Science, 164, 312, 1969(PMID: 5813351). |
| References by users | Mol. Cell Biol., 25, 4388-4396, 2005(PMID: 15899845)., J. Pept. Sci., 14, 261-266, 2008(PMID 17880034) |
| Comments | |
| Deposited by | Pharmacol. (Inst. Develop. Aging and Cancer, Tohoku Univ.). |
| Restriction | |
| Photo |   Photo(x100) Photo(x200) |