| ID |
TKG 0319 |
| Cell name |
M1(D+) |
| Data accepted |
Oct., 2, 1989. |
| Animal |
Mouse (SJ). |
| Scientific name |
Mus musculus. |
| Sex |
|
| Case history |
|
| Tissue |
Spontaneous myeloid leukemia of SJ mouse. |
| Genetics |
|
| Life span |
Infinite. |
| Morphology |
Lymphoblast-like. |
| Medium |
RPMI-1640 + 10% FBS. |
| Passage method |
Dilute 1/5 every 3-4 days. |
| Characteristics |
Model cell line for differentiation in the myeloid macrophage pathway.
This cell line can be induced to become macrophage-like by dexamethazone,
LPS and WEHI-3 supernatant. A culture submitted to the Cell Resource Center
for Biomedical Research was found to be contaminated with mycoplasma. Progeny
were cured by Treatments with BM Cyclin and MC210. |
| Temperature |
37℃ |
| CO2 concentration |
5% |
| Subculture frequency |
|
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Isozyme analysis |
LD |
| Established by |
Ichikawa, Y. (Kyoto U.).
|
| References |
J.
Cell. Physiol., 74, 223-234, 1969.PMID: 4187945 J.
Immunol., 130, 108-114, 1983.PMID: 6847876 |
| References by users |
|
| Comments |
|
| Deposited by |
Okuda, T. |
| Restriction |
|
| Photo |
 |
