| ID |
TKG 0318 |
| Cell name |
HMV-II |
| Data accepted |
Aug., 7, 1987.
|
| Animal |
Human. |
| Scientific name |
Homo sapiens. |
| Sex |
|
| Case history |
|
| Tissue |
Vagina melanoma (65 y. o.). |
| Genetics |
|
| Life span |
Infinite. |
| Morphology |
Spindle-like. |
| Medium |
F12 (or MEM/F12) + 10% FBS. |
| Passage method |
0.25% trypsin. |
| Characteristics |
Melanotic melanoma (highly melanotic). A culture submitted to the Cell
Resource Center for Biomedical Research was found to be contaminated with
mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
|
| Temperature |
37℃ |
| CO2 concentration |
5% |
| Subculture frequency |
1:1 ~ 1:2, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining; - |
| Established by |
Kasuga, T. (Prof. Dermatol., Tokyo Med. Dent. Collge).
|
| References |
Cell Differentiation, 1, 149-155, 1972(PMID: 4209534)., Jingansaibou no baiyo (Ohboshi and Sugano ed. Asakura Publisher, 1975)
pp. 203-208. Cancer, 42, 2675-2686, 1978(PMID: 365316)., |
| References by users |
Biochim. Biophys. Acta., 1009, 283-286, 1989(PMID: 2480811)., Chem. Pharm. Bull, 51, 798-801, 2003(PMID: 12843585)., Anticancer Res., 26, 4349-4356, 2006(PMID: 17201154). |
| Comments |
|
| Deposited by |
Saeki, H. (CCR. Tohoku Univ.) |
| Restriction |
|
DNA Profile
(STR-PCR) |
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
| 12 |
11 |
8, 14 |
11 |
16, 17 |
6, 9 |
X |
8 |
13 |
|
| Photo |
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