| ID |
TKG 0308 |
| Cell name |
BHK-21 |
| Data accepted |
|
| Animal |
Hamster (Syrian or golden) |
| Scientific name |
Mesocrcetus auratus |
| Sex |
|
| Case history |
|
| Tissue |
Kidneys of five unsexed, 1-day-old hamsters |
| Genetics |
|
| Life span |
Infinite |
| Morphology |
Fibroblast-like, pseudodiploi.
|
| Medium |
MEM + 10% newborn bovine serum (or FBS). |
| Passage method |
|
| Characteristics |
This cell line has been used for polyoma virus transformation, foot-and
mouth disease, rabies, and many arbovirus replication studies. A culture
submitted to the Cell Resource Center for Biomedical Research was found
to be contaminated with mycoplasma. Progeny were cured by Treatments with
BM Cyclin and MC210. |
| Temperature |
37℃ |
| CO2 concentration |
5% |
| Subculture frequency |
1:9, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Isozyme analysis |
LD |
| Established by |
Macpherson, I. A. & Stocker, M. G. P. (1961).
|
| References |
Virologoy, 16, 147, 1962. J. Natl. Cancer Inst., 30, 795, 1963.
|
| References by users |
|
| Comments |
|
| Deposited by |
Kan, M. (RITC, Tohoku U.).
|
| Restriction |
|
| Photo |
 (x100) |
