| ID |
TKG 0306 |
| Cell name |
293 |
| Data accepted |
|
| Animal |
Human. |
| Scientific name |
Homo sapiens. |
| Sex |
|
| Case history |
|
| Tissue |
Primary embryonal kidney (human). |
| Gentics |
|
| Life span |
Infinite. |
| Morphology |
|
| Medium |
Eagle's MEM + 10% FBS. |
| Passage method |
0.25% trypsin. |
| Characteristics |
Primary human kidney (embryonal) transformed by sheared human adenovirus
type 5 (Ad 5) DNA, contain and express the transfrming genes of Ad 5. Handle
as potentially biohazadeous material under Biosafety level 2. Excellent
for titrating human adenoviruses. A culture submitted to the Cell Resource
Center for Biomedical Research was found to be contaminated with mycoplasma.
Progeny were cured by Treatments with BM Cyclin and MC210. |
| Temperature |
|
| CO2 concentration |
|
| Subculture frequency |
|
| Classification |
|
| Mycoplasma |
PCR: - |
| Established by |
|
| References |
J. Gen. Virol., 36, 59-74, 1977(PMID: 886304)., Virology, 77, 319-329, 1977(PMID: 841862)., Virology, 86, 10-21, 1978(PMID: 664220). |
| References by users |
Br. J. Radiology, 75, 657-662, 2002(PMID: 12153939)., Nat. Methods, 7, 729-732, 2010(PMID: 20693999). |
| Comments |
|
| Deposited by |
|
| Restriction |
|
DNA Profile
(STR-PCR) |
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
| 8, 9 |
12 |
11, 12 |
9, 13 |
16, 19 |
7, 9.3 |
X |
11 |
11, 12 |
|
| Photo |
 |
