| ID |
TKG 0304 |
| Cell name |
KU812-F |
| Data accepted |
Nov., 1991. |
| Animal |
Human (38 y. o. male).
|
| Scientific name |
Homo sapiens.
|
| Sex |
|
| Case history |
|
| Tissue |
Blood (leukemia cells). |
| Genetics |
|
| Life span |
Infinite. |
| Morphology |
Lymphoblast-like (myeloblast-like), adherent.
|
| Medium |
RPMI-1640 + 10% FBS.
|
| Passage method |
Dilute 1/5 every 3-4 days. |
| Characteristics |
Human basophilic leukemia. In serum-free medium, this cell line differentiates
to basophilic cells. A culture submitted to the Cell Resource Center for
Biomedical Research was found to be contaminated with mycoplasma. Progeny
were cured by Treatments with BM Cyclin and MC210. |
| Temperature |
37℃ |
| CO2 concentration |
5% |
| Subculture frequency |
|
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Established by |
Kishi, K. (Niigata U.).
|
| References |
Leuk.
Res., 9, 381-390, 1985.PMID: 3858609 Leuk.
Res., 11, 1105-1113, 1987.PMID: 3695564 Blood,
69, 1414-1418, 1987.PMID: 2436686 Blood,
70, 612, 1987.PMID: 3304457 |
| References by users |
|
| Comments |
|
| Deposited by |
Fukuda, T. (Nigata U., Dep. Pathol.), Tanno, Y., Yamauchi, K. (1st. Dep.
Internal Med., Tohoku U.).
|
| Restriction |
|
DNA Profile
(STR-PCR) |
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
| 10 |
8 |
12 |
9 |
15 |
9 |
XY |
12 |
12, 13 |
|
| Photo |
 |
