| ID |
TKG 0302 |
| Cell name |
HMV-I |
| Data accepted |
Feb., 3, 1986. |
| Animal |
Human. |
| Scientific name |
Homo sapiens. |
| Sex |
|
| Case history |
|
| Tissue |
Melanoma (vagina origin).
|
| Gentics |
|
| Life span |
Infinite. |
| Morphology |
Epithelial-like.
|
| Medium |
DMEM/F12 + 10% FBS. |
| Passage method |
0.25% trypsin. |
| Characteristics |
Human melanoma, melanin production (-). Evidence from DNA fingerprinting
indicates that this line and HeLa are derived from the same individual. A
culture submitted to the Cell Resource Center for Biomedical Research was
found to be contaminated with mycoplasma. Progeny were cured by Treatments
with BM Cyclin and MC210. |
| Temperature |
|
| CO2 concentration |
|
| Subculture frequency |
|
| Classification |
|
| Mycoplasma |
PCR: - |
| Established by |
Kasuga, T.
|
| References |
Cell
Differentiation, 1, 149-155, 1972.PMID: 4209534 Biology of Normal and
Abnormal Melanocytes. (Kawamura ed., university Tokyo Press), pp. 241-264, 1971.
Jingansaibou no baiyo, pp. 388, 1975 (Asakura Press). Cancer,
42, 2675-2686, 1978.PMID: 365316
|
| References by users |
|
| Comments |
|
| Deposited by |
Oikawa, A. (Inst. Develop. Aging and Cancer, Tohoku Univ.). |
| Restriction |
|
DNA Profile
(STR-PCR) |
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
| 11, 12 |
13.3 |
8, 12 |
9 |
16, 18, 19 |
7 |
X |
8, 12 |
9, 10 |
|
| Photo |
 |
