| ID |
TKG 0299 |
| Cell name |
NIH/3T3 |
| Data accepted |
April, 1992. |
| Animal |
Mouse (Swiss).
|
| Scientific name |
Mus musculus |
| Sex |
|
| Case history |
|
| Tissue |
Embryonal fibroblast. |
| Genetics |
|
| Life span |
Infinite. |
| Morphology |
Fibroblast-like. |
| Medium |
DMEM + 10% calf serum.
|
| Passage method |
0.05% trypsin, 3 x 10^5 cells/6 cm dish, every 3 days. |
| Characteristics |
Contact inhibition (+), useful for oncogene study.
|
| Temperature |
37℃ |
| CO2 concentration |
5% |
| Subculture frequency |
|
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Isozyme analysis |
LD |
| Established by |
Todaro, G. J. |
| References |
J. Cell Biol., 17, 299-313, 1963(PMID: 13985244)., J. Virol., 4, 549-553, 1969(PMID: 4311790)., Oncologia (Jap.), 17, 47-57, 1986. |
| References by users |
Bioorg. Med. Chem. Lett., 14, 2563-2566, 2004(PMID: 15109652), J Vet Med Sci. 2023 Jun 1;85(6):592-600(PMID: 37150603). |
| Comments |
|
| Deposited by |
Yamamoto, T.
|
| Restriction |
|
| Photo |
 |
