| ID |
TKG 0294 |
| Cell name |
MG-63 |
| Data accepted |
May, 1992.
|
| Animal |
Human. |
| Scientific name |
Homo sapiens.
|
| Sex |
|
| Case history |
|
| Tissue |
Osteosarcoma.
|
| Genetics |
|
| Life span |
Infinite. |
| Morphology |
Epithelial-like. |
| Medium |
Eagle's MEM + 10% FBS or GIT.
|
| Passage method |
0.25% trypsin or 0.02% EDTA-PBS.
|
| Characteristics |
14 years old male, high yield of IFN production by induction with poly IC,
cyclohexmide and actinomycin D. Antigenically, MG-63 IFN is closely related to
human fibroblast IFN. |
| Temperature |
37℃ |
| CO2 concentration |
5% |
| Subculture frequency |
1:10 ~ 1:20, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Established by |
|
| References |
Antimicrob. Agents. Chemother., 12, 11-15, 1977(PMID: 883813). |
| References by users |
Int. J. Mol. Med., 5, 505-510, 2000(PMID: 10762653), Toxicology Letters, 143, 1-7, 2003(PMID: 12697374), Bone. 2007 Apr;40(4):876-87(PMID: 17254854), Cancer Sci. 2008 Mar;99(3):518-23(PMID: 18081879), Lite Sci. 2016 Jun 15:155:29-35(PMID: 27177772), Int J Mol Sci. 2017 Oct 17;18(10):2159(PMID: 29039776), Int Immunol. 2021 Jul 23;33(8):447-458(PMID: 34089617). |
| Comments |
|
| Deposited by |
Asakura, S. (Jichi Med. College). |
| Restriction |
|
DNA Profile
(STR-PCR) |
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
| 11, 12 |
11 |
10 |
11 |
16, 19 |
9.3 |
XY |
8, 11 |
10, 12 |
|
| Photo |
 |
