細胞株の詳細データ

TKG 0233 ：： NS-1(P3/NS1/1-Ag4-1)

ID	TKG 0233
Cell name	NS-1 (P3/NS1/1-Ag4-1)
Data accepted	Nov., 1980.
Animal	Mouse (BALB/c).
Scientific name	Mus musculus.
Sex
Case history
Tissue	B cell (myeloma), derived from MOPC-21.
Genetics
Life span	Infinite.
Morphology	Myeloma-like.
Medium	RPMI-1640 + 10% FBS.
Passage method	Dilute 1/8 every 3-4 days.
Characteristics	Parent myeloma to construct mouse B cell hybridoma, HGPRT(-). Intracellular kappa chains are only secreted in a hybrid cell when attached to heavy chain.
Temperature	37℃
CO2 concentration	5%
Subculture frequency
Classification
Mycoplasma	PCR: -, Hoechst DNA staining: -
Isozyme analysis	LD
Established by	Kohler, G. & Milstein, C.
References	Eur. J. Immunol., 6, 511, 1976.PMID: 825377 J. Mol. Biol., 60, 61, 1970. Exp. Cell. Res., 60, 61, 1970.PMID: 5439579
References by users
Comments
Deposited by	Uno, M. (Nihon Univ.).
Restriction
Photo	Photo(X200)