| ID |
TKG 0206 |
| Cell name |
HuH-7 |
| Data accepted |
|
| Animal |
Human. |
| Scientific name |
Homo sapiens. |
| Sex |
|
| Case history |
|
| Tissue |
Liver.
|
| Genetics |
|
| Life span |
Infinite. |
| Morphology |
Epithelial-like.
|
| Medium |
RPMI-1640 + 10% FBS (30% conditioned med) or GIT.
|
| Passage method |
0.25% trypsin. |
| Characteristics |
Highly differentiated, hepatocelluler car., AFP+, ALB+, C3, C4.
|
| Temperature |
37℃ |
| CO2 concentration |
5% |
| Subculture frequency |
1:2 ~ 1:4, twice a week. |
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Established by |
Nakabayashi, H.
|
| References |
Cancer Res., 42, 3858-3866, 1982., Cancer Res., 45, 6379-6383, 1985(PMID:
2415243)., Tiss. Cul. Res. Commun., 12, 221-227, 1993. |
| References by users |
J. Hepatolo., 27, 677-687, 1997(PMID: 9365044)., Hepatology, 33, 1087-1097, 2001(PMID: 11343236)., Mol. Cell Endocrinol., 231, 75-85, 2005(PMID: 15713537), Toxicol Lett. 2015 Jan 22;232(2):393-402(PMID: 25448278), Int J Mol Med. 2017 Sep;40(3):748-754(PMID: 28677736), Chem Pharm Bull. 2018;66(6):682-687(PMID: 29863070), Cell Mol Gastroenterol Hepatol. 2018 Sep 11;7(1):115-134(PMID: 30510994). |
| Comments |
|
| Deposited by |
Tanno, H. (1st Dept. Surg., Tohoku Univ.).
|
| Restriction |
|
DNA Profile
(STR-PCR) |
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
| 12 |
10,11 |
11 |
10 |
16,18 |
7 |
X |
8,11 |
11 |
|
| Photo |
 |
