細胞株の詳細データ

TKG 0184 ：： A549

ID	TKG 0184
Cell name	A549
Data accepted
Animal	Human.
Scientific name	Homo sapiens.
Sex
Case history
Tissue	Lung.
Genetics
Life span	Infinite.
Morphology	Epithelial-like.
Medium	RPMI-1640 + 10% FBS (MEM +10% FBS + NEAA).
Passage method	0.25% trypsin.
Characteristics	Adenocarcinoma. HLA-A 2603/3001. A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
Temperature	37℃
CO2 concentration	5%
Subculture frequency	1:10 ~ 1:20, twice a week.
Classification
Mycoplasma	PCR: -, Hoechst DNA staining: -
Established by	Giard, D. J. et al.
References	J. Natl. Cancer Inst., 51, 1417-1423, 1973(PMID: 4357758).
References by users	Int. J. Radiat. Oncol. Biol. Phys., 1171-1176, 1998(PMID: 9719129)., Biochem. Biopys. Res. Commun., , 270, 649-656, 2000(PMID: 10753678)., Toxicology Letters, 143, 1-7, 2003(PMID: 12697374)., Bioorg. Med. Chem. Lett., 14, 2563-2566, 2004(PMID: 15109652)., Bioorg. Med. Chem. Lett., 15, 2966-2969, 2005(PMID: 15914002)., Chem. Biodivers., 2, 689-694, 2005(PMID: 17192012)., Cell Biol. Int., 31, 1518-1524, 2007(PMID: 17719804)., FEBS. Lett., 581, 5649-5657, 2007(PMID: 18022393)., Cancer Res., 70, 6659-6669, 2010(PMID: 20710045), Cancer Med. 2013 Jun;2(3):305-15(PMID: 23930207), Autophagy. 2014 Mar;10(3):497-513(PMID: 24384561), Chem Pharm Bull. 2018;66(6):682-687(PMID: 29863070), Tohoku J Exp Med. 2019 Apr;247(4):215-222(PMID: 30971638), Sci Rep. 2025 Jul 3;15(1):23790(PMID: 40610626), Mol Biol Rep. 2025 Nov 14;53(1):81(PMID: 41238969), Respir Investig. 2026 Jul;64(4):101471(PMID: 42314620).
Comments
Deposited by
Restriction
DNA Profile (STR-PCR)	D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO 11 11 8, 11 11, 12 14 8, 9.3 XY 8, 11 10, 12
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