| ID |
TKG 0154 |
| Cell name |
L1210 |
| Data accepted |
|
| Animal |
Mouse (DBA/2) |
| Scientific name |
Mus musculus. |
| Sex |
F. |
| Case history |
MCA |
| Tissue |
Spleen lymph node, leukemia. |
| Genetics |
|
| Life span |
Infinite. |
| Morphology |
|
| Medium |
RPMI-1640 + 10% Horse serum or RPMI-1640 + 10% FBS. |
| Passage method |
Dilution. |
| Characteristics |
This tissue culture cell line was established from a
cell line maintained by in vivo transplantation. Conditions for transplantation, ip:100:7-10 (transplantation site : transplantation
rate : survival days). Culture cell line was found to be contaminated with
mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
|
| Temperature |
37℃ |
| CO2 concentration |
5% |
| Subculture frequency |
|
| Classification |
|
| Mycoplasma |
PCR: -, Hoechst DNA staining: - |
| Isozyme analysis |
NP |
| Established by |
|
| References |
J. Natl. Cancer Inst., 10, 179-192, 1949(PMID: 18143260)., Cancer Res., 20, 930-939, 1960(PMID: 13823721)., Cancer Chemother. Rep., 51, 451-453, 1967. |
| References by users |
Int. J. Rad. Appl. Instrum. B, 16, 371-374, 1989(PMID: 2777577)., Int. J. Rad. Appl. Instrum. B, 363-367, 1990(PMID: 2387744). |
| Comments |
From NCI.
|
| Deposited by |
|
| Restriction |
|
| Photo |
 x100 |
